Lab Techniques
Equipment and techniques demonstrated here are either in our lab or other labs we collaborate with.
"IHC is an antibody application which allows you to visualize proteins in tissue while retaining its microstructure...allows for comparison between healthy and diseased tissues." - ptglab.com
"This is a method to purify DNA by using physical and/or chemical methods from a sample separating DNA from ... other cellular components." -NCBI
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"PCR means polymerase chain reaction. It's a test to detect genetic material from a specific organism, such as a virus." - Cleveland Clinic
"Platelet aggregation measures the ability of platelets to adhere to one another and form a hemostatic plug, which is the key component of primary hemostasis." - Science Direct.
Immunohistochemistry
DNA & RNA Extraction
PCR
Platelet Aggregation
"The fibrinogen blood test is used to assess how well the protein called fibrinogen — also called coagulation factor I — performs in the blood and to measure its levels in your blood." - WebMD
"Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms." - Wikipedia
"The diagnosis and study of placental diseases of the tissues, and involves examining placental tissues and/or cells under a microscope" - Royal College of Pathologists
"DNA sequencing is a laboratory technique used to determine the exact sequence of bases (A, C, G, and T) in a DNA molecule." - National Human Genome Research Institute
Fibrinogen Levels
Molecular Cloning
Placenta Histopathology
DNA Sequencing
"Flow cytometry is a technology that rapidly analyzes single cells or particles as they flow past single or multiple lasers while suspended in a buffered salt-based solution." - NCBI
We used this to understand the procoagulant role of microparticles in cancer and as markers for endothelial, platelet function
Tissue factor (TF) is the the primary physiologic activator of the blood coagulation system. we measured TF in plasma and in placental tissue
Check out Dr. Othman's paper "Platelet-type Von Willebrand disease: three decades in the life of a rare bleeding disorder"
Flow Cytometry
Microparticles Assays
Tissue Factor Assays
PT-VWD Mouse Model Studies
"Partial thromboplastin time (PTT) and activated partial thromboplastin time (aPTT) are used to measure and evaluate all the clotting factors of the intrinsic and common pathways of the clotting cascade" - MedScape
"RT-PCR (reverse transcription-polymerase chain reaction) is the most sensitive technique for mRNA detection and quantitation currently available." - ThermoFisher
Pregnant mouse model studies are done in collaboration with Dr Charles Graham Queen's
"Confocal microscopy uses light from a laser through the objective of a standard light microscope to excite a specimen within a narrow plane of focus" - Ohio Wesleyan University
PT, aPTT
RT-PCR
Pregnant Mouse Model Studies
Confocal Microscopy
"Cell culture refers to the removal of cells from an animal or plant and their subsequent growth in a favorable artificial environment." - ThermoFisher
Cell Culture and Protein Expression
"Intravital microscopy is a powerful imaging tool for visualizing various biological processes, simultaneously. This allows for in vivo study of several critical cell types..." - News Medical
Intravital Microscopy
​Other Techniques
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Basic, special coagulation tests, molecular biology techniques​​​
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E1-E3 deleted adenovirus – HEK 293 cell growth
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Fibrin degradation products (d.dimer)
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Plasminogen, t-PA and PAI (to evaluate fibrinolysis)